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CRISPR/Cas9 Products

Genome editing technology is now widely used for making gene knockout/knock-in animals and plants as well as genome-modified cell lines. KRISHGEN provides three types of genome-editing protein: wtCas9, eSpCas9(1.1), AsCpf1 that exhibit higher efficiency, specificity and undetectable off -target effect.

Cas9 protein is a purified nuclease from E coli using Cas9 gene of Streptococcus pyogenes. It is a ready-to-use product containing a nuclear localization signal (NLS) for genome editing experiments resulting site-specific cleavage of double stranded DNA

Key Features of the Genome Editing Proteins:

Product Cas9 Protein eSpCas9(1.1) AsCpf1 Protein
Cat No KB-cas9-50ug/500ug/1mg KB-cas901-50ug/500ug/1mg KB-cpf1-50ug/500ug/1mg
Off-target Effect Low Not Detectable Not Detectable
Efficiency Excellent Good Good
Specificity Good Excellent Excellent
PAM Site NGG NGG TTTN
mRNA/td> sgRNA sgRNA crRNA
Size 50 ug / 500 ug / 1mg 50 ug / 500 ug / 1mg 50 ug / 500 ug / 1mg

High Activity - Cas9 Protein:


KB-SGR50 | sgRNA (sgRNA mRNA is chemically synthesized and ready-to-use product containing a custom-designed sgRNA for excellent efficiency and specificity. Along with Cas9 protein or eSpCas9 (1.1) protein these ready-to-inject reagents are suitable for producing gene-editing animals via one-cell embryos injection. This product can also be used to directly cut the target sequence in vitro)


KB-CRR20 | crRNA (crRNA is chemically synthesized and ready-to-use product containing a custom-designed sgRNA for excellent efficiency and specificity. Along with AsCpf1 protein (these ready-to-inject reagents are suitable for producing gene-editing animals via one-cell embryos injection. This product can also be used to directly cut the target sequence in vitro)


KB-cas9-50ug/500ug/1mg | wtCas9 Protein (see typical lot release data)


KB-cas901-50ug/500ug/1mg | eSpCas9(1.1) Protein (see typical lot release data)


KB-cpf1-50ug/500ug/1mg | AsCpf1 Protein (see typical lot release data)

    Total 11 products.

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