Background: Ampicillin belongs to the beta-lactams family, which once played very important role in preventing and curing animal diseases. For it causes anaphylactic reaction and resistance, in EU, US and China, it is being restricted. The common instrumental analysis of this drug is limited because of the complicated operation and high expense, while this kit is a new product based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 1.5h in single detection, so it can considerably minimize operation error and work intensity.This ELISA can be used in the production of therapeutic drugs and vaccines, to accurately quantify drug residues and ensure quality and purity of the final product. Current method of drug residue testing such as HPLC or GC-MS are with sample prep not only complex and expensive, but also difficult and time consuming. ELISAs speed up and simplify the analysis without losing accuracy.
Principle: KRIBIOLISA Ampicillin ELISA kit is based on indirect-competitive ELISA technology. The microtiter wells are coated with Ampicillin antigen. Ampicillin residue in the sample competes with the antigen coated on the microtiter plate for the antibody. After the addition of enzyme labeled anti-antibody, TMB substrate is used to show the color. Absorbance of the sample is negatively related to the ampicillin reside in it, after comparing with the Standard Curve, multiplied by the dilution factor, ampicillin residue quantity in the sample can be calculated.
