Transmembrane Protein ELISA
The study of transmembrane proteins is a dynamic and rapidly evolving field. Researchers are continuously exploring their structure, function, and interactions to gain insights into fundamental cellular processes and identify novel therapeutic opportunities. The accurate detection, quantification, and analysis of transmembrane proteins using techniques like ELISA are essential for advancing our understanding of their impact on cellular biology, disease mechanisms, and the development of innovative treatments.
Krishgen has recently launched our range of KRIBIOLISA Transmembrane proteins ELISA for the accurate measurement of these protein levels. Accurate measurement of transmembrane protein levels using our ELISA kits allows researchers to study receptor dynamics, ligand-receptor interactions, and downstream signaling pathways, unraveling the intricacies of cellular communication.
- All validation, verification and lot releases for these ELISA are performed according to best-in-class scientific guidelines – including a stringent seven-point validation to ensure quality and robustness lot-over-lot.
- Extensive validation of the kit with details provided.
- These ELISA employ humanized monoclonal antibodies in a sandwich assay format, ensuring exceptional specificity and sensitivity in drug detection, even at low concentrations.
- Unlike other assay available commercially, only KRIBIOLISA assays offer humanized capture antibodies. This leads to a high degree of specificity and sensitivity to the assays.
- Standards / calibrators offered in the kit are validated against recombinant proteins.
- Validated and manufactured in facility certified under ISO 13485.
- 96 well plates with break apart strips/wells helps in running smaller sample lots.
- Includes lyophilized standards for greater stability. Reduced chances of degradation of components during shipping with low maintenance of cool chain. Shipped at ambient temperature and to be stored at 2-8^C on receipt.
- Kit expiry period of a minimum 10 months available.
Performance Characteristics and Validation Information:
Sensitivity: The limit of detection is defined as the lowest detectable concentration corresponding to a signal of Mean of ‘0’ standard plus 2* SD. Each kit mentions sensitivity in the datasheet.
Calibration: The Standards provided in the kit are calibrated against commercially sourced recombinant proteins.
Recovery and Matrix Effect:
The kits have been optimized at specific dilution to ensure optimal noise:signal ratios. Please do not change the dilution of samples recommended in the IFUs. For any queries, please connect with us directly.
Precision: Precision is defined as the percent coefficient of variation (%CV) i.e. standard deviation divided by the mean and multiplied by 100. Assay precision was determined by both intra and inter assay reproducibility on two pools with low, medium, and high concentrations. While actual precision may vary from laboratory to laboratory and technician to technician, it is recommended that all operators achieve precision below these design goals before reporting results.
Customization Available
Require an ELISA for a transmembrane protein that isn’t available commercially yet? Our services team can custom develop an assay for you. Talk to our team for a free technical feasibility consultation today.
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Data Reduction: We typically use linear graphs and 4PL (2nd order) as the data reduction method. Cubic spline is also recommended in our IFUs. Our typical graph shows the graph in 4PL and also indicates the R2 value with the trend line shown.
Transmembrane Protein ELISA
Transmembrane proteins play a crucial role in various biological processes and have a significant impact on cellular function, signaling, and overall organismal physiology.
They serve as receptors and channels, allowing cells to communicate with their external environment; recognize and bind specific ligands, such as hormones, growth factors, or neurotransmitters, initiating intracellular signaling cascades that regulate gene expression, cell growth, differentiation, and other critical cellular responses. Transmembrane proteins also regulate the movement of ions and molecules across cell membranes and have functions in signal transduction.
Playing a fundamental role in relaying extracellular signals to the interior of cells, these proteins initiate a cascade of molecular events. They are often implicated in various diseases, including cancer, cardiovascular disorders, neurological conditions, and genetic disorders. Mutations or dysregulation of transmembrane proteins can disrupt normal cellular processes, leading to disease progression. Understanding their role and function is essential for the development of targeted therapies and diagnostic tools. Additionally, due to their involvement in disease pathways and their accessibility at the cell surface, transmembrane proteins serve as attractive targets for drug development. Modulating the activity or expression of these proteins with targeted therapies, such as monoclonal antibodies or small molecule inhibitors, holds promise for treating a wide range of diseases.
If you are unable to locate your desired ELISA kit on our website, or have a technical query, please drop us a message and our team will come back to you with availability. If we do not have it available, we will suggest a feasible custom development option.
Require a Custom Assay?
Krishgen can develop and manufacture to your specific requirements.
Whether you require a change in the assay range or sensitivity, want an assay validated for a specific matrix or even want an novel immunoassay developed, the Krishgen team can support you. Get a free technical consultation to learn about the feasibility and timeline of your new assay.