Background: Erythromycin residue in the production of biological products may lead to abnormal reactions of human beings, thus strict MRLs have been established. This kit is a rapid test product for the determination of tetracycline residues which is sensitive, accurate and time saving. It can considerably reduce the operation errors in the assay.
Principle: This kit uses Indirect Competitive-ELISA as the method. It can detect Erythromycin residue in biological samples. The microtiter wells are precoated with capture BSA-linked antigen. Samples and standards are pipetted into microwells and Erythromycin present in the sample will competes with antigen coated on the microtitre plate for the antibody. Then enzyme conjugate is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops inversely proportionally to the amount of Human Erythromycin in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
