Introduction: Human IgG is a monomeric immunoglobulin, and the most abundant one constituting approximately 75% of the serum. Presence of such trace contaminants in the products may lead to potential health hazards, especially when the product is intended for therapeutic use. Intended Use: This generic kit is intended in determining the presence of Total human Immunoglobulin G contamination in variety of sample types. A highly sensitive and specific Enzyme linked immunosorbent assay can be used for the quantitation of low levels of human IgG.
Principle: This assay is based on the Sandwich ELISA procedure. Samples containing hIgG are reacted with anti hIgG-HRP antibody simultaneously in the microtiter wells already coated with affinity purified capture IgG antibody. This immunological reaction results in formation of a sandwich complex of solid phase antibody-hIgG-enzyme labeled antibody. The wells are washed to remove any unbound reactants as per the wash procedure (see Assay procedure section mentioned below). The substrate 3, 3, 5, 5 Tetramethyl Benzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the concentration of hIgG present.
