Introduction:
Serratia marcescens nuclease originates from Gram negative bacteria S. marcescens and heads a family of homological non-specific nucleases that are widely spread in the world. Serratia nuclease is most studied one and it is capable to cleavage both RNA and DNA in either single or double stranded form.
Intended Use:
The Endonuclease Serratia marcescens ELISA kit developed for quantitative determination of endonuclease in samples from downstream processing where endonuclease is used as a process or purification aid.
Principle:
The Endonuclease Serratia marcescens (FastNuclease) ELISA is a competitive immunoassay for the determination of Serratia endonuclease in sample. The secondary antibodies specific to anti-endonuclease are coated on to the microtiter plate. A constant concentration of HRP labeled endonuclease and varying concentration of standard or sample containing endonuclease compete for binding to anti-endonuclease antibodies. This immune complex is captured by the coated secondary antibody. After incubation and washing, the unbound labeled enzyme is removed. Then addition of substrate develops blue color during incubation period and the reaction is stopped after the addition of stop solution with development of yellow color. The intensity of the color generated is inversely proportional to the amount of endonuclease in the sample.
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