Krishzyme Endo-beta-N-acetylglucosaminidase S (Endo S) (lyophilized)

Highly specific for human antibodies Krishzyme Endo S is an endoglycosidase with a unique accuracy for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG molecules. Specifically, this endo-beta-N-acetylglucosaminidase S hydrolyzes the beta(1-4) linkage between the two core GlcNAcs of asparagine linked biantennary complex-type glycans of human IgG Fc regions. This enzyme will leave any human IgG with a single N-Acetylglucosamine, with or without an attached fucose molecule. This recombinant glycosidase is cloned from Streptococcus pyogenes, and is meticulously purified for maximum activity without contaminating bio-molecules or proteins. Like other Krishzyme glycosidases, Endo S is lyophilized for stability during shipment and storage. Its performance is outstanding with its ability to produce clean peaks with very high yields (as detected by HPLC), and is commonly used in drug development or other research that is highly focused on the use of human antibodies. This endoglycosidase only deglycosylates IgG glycoforms and plays a central role in glycoengineering strategies for the development of IgG antibodies with improved therapeutic efficacy.