Background: Cannabinoid receptor type 2 (CB2) is a G protein-coupled receptor primarily expressed in immune cells, peripheral tissues, and the central nervous system. CB2 plays a crucial role in modulating immune responses, inflammation, pain perception, and neuronal function. Dysregulation of CB2 signaling has been implicated in various disorders, including inflammatory diseases, neurodegenerative conditions, and chronic pain syndromes. The Human CB2 ELISA Kit offers a reliable method for quantifying CB2 receptor levels in human serum, plasma, and tissue samples, facilitating studies aimed at understanding receptor function, disease mechanisms, and therapeutic interventions. By quantifying CB2 receptor levels, researchers can gain insights into immune regulation, neuroprotection, and pain modulation. Furthermore, aberrant CB2 expression or activity is associated with conditions such as autoimmune diseases, neuropathic pain, and psychiatric disorders. Understanding CB2 pathways is crucial for developing targeted therapies aimed at modulating cannabinoid receptor function and mitigating disease progression. Ongoing studies are investigating the molecular mechanisms underlying CB2 receptor regulation, its interactions with other signaling pathways, and its therapeutic potential for treating various disorders. Additionally, researchers are exploring the use of CB2 receptor agonists and antagonists as potential therapeutic agents for immune modulation, neuroprotection, and pain management. The Human CB2 ELISA Kit is an invaluable tool driving innovative research and paving the way for personalized interventions in immunology, neurology, and pain medicine.
Additional information
| Target / Pathway | Tumor associated antigen |
|---|---|
| Active Molecule / Modality | Protein target (solid/hematologic tumors) |
| Pack Type | 1 x 96 wells, break-apart |
| Species Reactivity | Homo Sapiens (Human) |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. For tear samples, please connect with us to discuss further. Note the kit has been validated using human matrices only. For any other animal matrix, pleas connect with us to optimize the kit accordingly. |
| Analyte / Drug | Human |
| Species / Population Reported for Cmax Value | not available |
| Dose & Regimen Reported | not available |
| Cmax (value + units) | Not reported in label/PI |
| Cmax Source (DOI/URL), where available | not available |
| Sample Dilution | Samples are to be diluted 1:1000 with the Sample Diluent provided in the kit. For more specific dilution instructions, please refer to the most updated IFU enclosed with the kit supplied |
| Assay Range Correlated with Cmax Value, where available | 0 – 2000 ng/ml |
| Standards Available | 0; 31.25; 62.5; 125; 250; 500; 1000; 2000 ng/ml |
| Calibration Curve | 6/8 point; standards available |
| Sensitivity | 15 ng/ml |
| Interference | Preparations of the known factors were assayed for interference. No significant interference was observed. |
| Specificity | The assay uses anti idiotypic (humanized) antibody for capture and / or recombinant protein expressed in mamalian cells, The standards used are research grade biosimilars referenced to the innovator product. In specfic cases where vailable the kit has also been validated against the innovator / reference product. Refer our detailed validation guide for the same. |
| Detection Method | Colorimetric, Absorbance measured at 450nm |
| Conjugate-Enzyme Reaction | Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development. |
| Shipping Temperature | Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius |
| Storage Temperature | The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity. |
| ELISA Type | Sandwich ELISA, Double Antibody |
| ELISA Interpretation | Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results. |
| Shelf Life Available | 8 -10 months at time of shipping |
| Alternate Names / Synonyms | Human CB2 |
| Research Area | Immune Molecule; Cell Biology; Immunology, Tumor |
