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ELISA Calculator Instructions

Tired of spending hours manually crunching ELISA data, prone to errors and frustrated by the lack of actionable insights? Wondering how to calculate ELISA results in Excel? Say goodbye to the tedious calculations and hello to streamlined analysis! Introducing our online calculator and Excel add-on, the perfect tools for researchers to unlock the full potential of your ELISA data.
Access our Online ELISA Results
Calculator and Download Excel Add On here.

Web or Cloud

Use curve fitting on your computer or on a page, Calculation are performed in the cloud

Without Internet

Calculation are completely offline with the use of a USB pen drive

Features & Benefits of the ELISA Calculator Online:

  • Effortless Interface: No software download or install needed, simply access the calculator from any web browser. 
  • Intuitive Analysis: Customize your analysis with pre-loaded formulas or create your own for complete control. 
  • Accurate Calculations: Eliminate manual errors and trust the power of our advanced algorithms. 
  • Seamless Data Export: Download your results in various formats for further analysis or publication. 
  • Time Saving: Fast, automated analysis saves time when running several rounds of experimental data.

Prefer working Offline? Use our ELISA Results Calculator Excel Add-On Instead:

  • Integrate Seamlessly: Work directly within your familiar Excel environment for smooth data flow. 
  • Automate Calculations: Let the add-on do the heavy lifting, automatically calculating results as you enter data. 
  • Visualize Your Data: Generate clear and informative graphs to quickly identify trends and patterns. 
  • Share & Collaborate: Easily share your analyzed data with colleagues or export for presentations.

Need Support?
Contact Our Tech Team

Ready to experience the power of streamlined ELISA analysis?

Frequently Asked Questions:

Yes, the ELISA calculator is free to use per person upto 15 times per day.

At this time, you can only order offline and not via the website.

You can run various functions including 2PL, 3PL, 4PL and others.

In case your lab isn’t connected to the internet, download the excel add-on onto a USB or your server, and download it

onto your computer. This is a one-time download, and you can use it upto 15 times per day for free without usage of the internet.

Yes, you can use a csv file – simply upload the relevant data using the “Import from CSV” button. In case your file is saved as an excel file (.xml or .xls etc), convert to a .csv file before uploading.

  • The use of Assayfit Pro in Microsoft Excel for Mac OS and Windows is easiest using the Add-in. The Excel add-in can be downloaded from our ELISA calculation page here. To install the add-in follow the following procedure:

  • After downloading the zip file you can copy the add-in somewhere on your hard drive or network. Use a location which is writeable. As an example we made a folder C:\add-in and copied the KrishgenAddin.xlam to this location. (You may need to set the security settings for Excel for this folder to trust the folder.)

  • To install, open excel and select options from the menu on Windows. On Mac OS select Excel add-ins from the Tools menu. You can also check this link https://support.office.com/en-us/article/Add-or-remove-add-ins-inExcel-0af570c4-5cf3-4fa9-9b88-403625a0b460#OfficeVersion=Window

  • In Windows follow the following menus Click Add-ins Press the go button in the bottom of the windows Then click browse and select the add-in.

  • Once it is installed a Menu named KRISHGEN appears. If you click the menu all KRISHGEN buttons are visible.

  • By clicking the right ELISA button a curve fitting sheet will appear with experimental data.

  • In the top table called input you can paste your data in 96 well format. In the second table you can identify where in the 96 well plate calibrators, controls, blanks and samples are located. This can be performed by typing or copying the identifies in the table or by using the plate layout builder.

We cover a broad spectrum of ELISA assays, including quantitative, qualitative, and competitive formats. Check our

website for a detailed list of supported assay types.

Subtracting the blank signal or normalize From the left menu or from the Options pull down menu you have several

options under Signals to modify the signals. Subtract blank will subtract the average of the samples marked with ID

“Blank” from all the samples and calibrators. Subtract CAL-0 will subtract the average signal of the calibrators marked

with ID “CAL-0” from all the samples and calibrators. Normalize will set the average of signals of samples marked with a

specified ID to 100% and will then calculate how the signals relate to this sample.

The graph the axis can be displayed as linear or logarithmic.

Yes.

You can analyse data for non-ELISA assays as well. Simply choose the current function while running the results.

You can select “save” to save the data in html format. You can also choose, for CSV or PDFoutput or choose to print.

You can set the sample dilution in the column marked Dil. This can be performed manually or by a copy paste action, but you can also assign the dilutions from the menu. There is a purple pull down menu on the left side of the page. Alternatively the top menu or the button marked options shows a similar menu. By selecting dilutions from the list and then one of the options, the selected dilutions will be entered for all of the samples in the list.

Alternatively you can select the positions of the dilutions to be changed and then click one of the dilution buttons above the table. Then only the selected cells will be filled with the selected dilution factor.

Yes.

Still have questions? Get in touch with our team at kbiinfo@krishgen.com and we will be happy to assist you with the online or offline ver

Disclaimer
The Krishgen Online ELISA Calculator service (use of the servers with address containing AssayFit.com and AssayCloud.com and cloud software on these servers) and any provided templates as well as custom templates (referred to as “Software or Service” in this Agreement) or offline software are the property of AssayCloud. By downloading, installing or using this Software or Service or parts of this Software or Service, you (or you on behalf of your organization) are agreeing to the terms and conditions of this Agreement. The Software and Service are protected under International Copyright Laws.

Changes in disclaimer and privacy policy
Krishgen has the right to change the disclaimer and privacy policy at any time Use of Krishgen Online ELISA Calculator

The free version and paid versions allow the use of the Software and/or Service by anyone provided they agree with the license agreement and privacy policy. By using the software, you agree with the license agreement and privacy policy.

Termination
This Agreement takes effect upon your use of the Software and Service and remains effective until terminated. It will be terminated automatically without notice from Krishgen if you fail to comply with any provision contained here. Krishgen has the right to exclude you from service upon misuse of the Service or Software associated with them. You agree on the right of Krishgen to terminate this Agreement.

Limitation of liability
In no event will Krishgen be liable to you or your company for any damages, including any lost profits, lost savings, or other incidental or consequential damages arising from the use or the inability to use the Software and/or Service, even if an authorized dealer or distributor has been advised of the possibility of these damages.

Changes in the conditions
At any time Krishgen can change the features and conditions of service of the Free API keys. You agree with the right of Krishgen to change the features and conditions. Krishgen will not change the conditions of the paid API keys for the period it has been purchased, except when applying an upgrade.

Warranty Disclaimer
Krishgen disclaims all warranties, whether expressed, implied, or otherwise, including the warranties of merchantability or fitness for a particular purpose. Krishgen does not warrant that the software is error-free or will operate without interruption.

Intended use
For Research Use Only. Not for use in diagnostic procedures

Privacy and personal data
At Krishgen, we take your privacy very seriously. In order to provide access to the service we must collect and store some personal information about you.

Minors
Children under 16 years of age are not permitted to access the Service or Software. By agreeing to this privacy policy, you are also agreeing that you are 16 years of age or older.

What is collected?
Basic profile information is collected from you when you create your account including your full name, address, phone number, IP address and email address. Your private information is stored on a single secure server at the AssayCloud.com domain with local hard drive backups. Your email address will be used on other servers linked to the Service and Software.

As you use the Service and Software, information about the services you interact with will also be stored and linked to your profile information. These will be used for account verification, monitoring of use and user credits, technical optimization, and marketing purposes. Statistics about pages you visit is collected using the outside services of Google analytics and Statcounter and is used for optimization of the site and marketing purposes. Your IP address and pages visited will be shared with Google and Statcounter and not the information provided using registration.

CookiesThe websites and services on the krishgen.com, krishgen.biz and krishgenbiosystems.com domains use cookies. Cookies are used for storing login information, user authorization and user statistics and for storing temporary data and saving settings. You agree to the use of cookies on these websites.

How is the information used?
This information is only used to provide you access to the services provided by Assayfit.com on any of the related servers and to improve the technical performance of the calculations provided by Assayfit.com and the site performance of servers with address containing krishgen.com. Statistical information about usage of the site is also used to improve the site and services provided on the website and related servers and for marketing purposes.

Whom to contact?
For any issues, questions and requests regarding the software, you can contact Krishgen at kbiinfo@krishgen.com

Datasharing
Your data is not shared with any other company except Krishgen and AssayCloud. Your personal data is stored as long as you are registered with www.AssayCloud.com and have not requested your data to be removed from the AssayCloud database.

Withdraw of consent for AssayCloud to store and process personal data

If you have previously consented to allow AssayCloud.com to store and process your personal data in accordance with this privacy policy, and you wish to withdraw your consent, please send an email to info@AssayCloud.com. You will no longer be able to access the services provided by www.assayfit.com if your consent is withdrawn.

Request that personal data is corrected or erased

You may make corrections to your basic profile information by logging into the www.AssayCloud.com and editing your own profile. If you have questions, or would like any other data to be corrected or erased, please send an email info@krishgen.com.

Request a record of all personal data that has been collected.

You may request a record of all of the personal data relating to you that has been collected in accordance with this privacy policy. To do so, please send an email to info@krishgen.com.com.

All rights reserved; 2024 by Krishgen Biosustems.

This javascript templates of Asssayfit Pro use Handsontable and Plotly. The use of Handsontable and Plotly are bound to the MIT license.

Copyright (c) 2012-2014 Marcin Warpechowski Copyright (c) 2015 Handsoncode sp. z o.o.

Copyright 2012-2018, Plotly, Inc. All rights reserved.

Licensed under the MIT license MIT license:

Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the ‘Software’), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:

The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software.

THE SOFTWARE IS PROVIDED ‘AS IS’, WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS.