Krishzyme Vaccinia virus Capping Enzyme is derived from a recombinant E. coli strain which carries the genes for the Vaccinia capping enzyme. This single enzyme is composed of two subunits (D1 and D12) and has three enzymatic activities (RNA triphosphatase and guanylyl transferase by the D1 subunit and guanine methyltransferase by the D12 subunit). Vaccinia virus Capping Enzyme is effective to catalyze the formation of cap structure, which can specifically attach the 7-methylguanylate cap structure (m7Gppp, Cap 0) to the 5 end of RNA.
Cap structure (Cap 0) plays an important role in mRNA stabilization, transport, and translation in eukaryotes. Capping RNA by the enzymatic reaction is an effective and simple method which can significantly improve the stability and translation of RNA for in vitro transcription, transfection, and microinjection.
Unit Definition
One unit of Krishzyme Vaccinia virus Capping Enzyme is defined as the amount of enzyme required to incorporate 10 pmol of GTP into an 80 nt transcript in 1 hour at 37 C.
Application
– Capping mRNA prior to translation assays/in vitro translation
– Labeling 5 end of mRNA