Introduction:
The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be
serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA
technique which leads to a higher specificity and increased sensitivity compared to conventional competitive
ELISA kits which employ only one antibody. Double antibodies are used in this kit.
Intended Use:
The GENLISA Human COL4A2, Collagen Alpha-2(IV) Chain ELISA kit is used as an analytical tool for
quantitative determination of Human COL4A2, Collagen Alpha-2(IV) Chain in serum, plasma and other biological
samples.
Principle:
The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells.
Samples and standards are pipetted into microwells and Human COL4A2, Collagen Alpha-2(IV) Chain present in
the sample are bound by the antibodies. Biotin labeled antibody is added and followed by Streptavidin:HRP is
pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding,
the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human
COL4A2, Collagen Alpha-2(IV) Chain in the sample. Color development is then stopped by addition of stop
solution. Absorbance is measured at 450 nm.
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