Validation of an ELISA for quantitative determination of Mouse SARS-COV-2 IgG antibodies using clinical samples as a tool for vaccine development. Authors: Krisha Jain, Atul Gadhave, Anitha Florance, Dr. Kalpesh Jain, Dharmendra Singh Abstract: Since the spread of SARS-CoV-2 in 2019 and the ensuing pandemic that led to a global halt, the race to develop […]
Technical Note: Validation of Krishgen ELISA for Accurate, Reproducible Results Krishgen ELISA are always fully validated using a stringent, thorough protocol that guarantees quality and performance. They are designed and developed to meet high standards set by the best manufacturers around the world. In this blog, learn more about our validation process. Optimization Optimization of […]
Technical Note: Use of anti-idiotypic antibodies in ELISA and a review on the better specificity offered Background: KRISHGEN Therapeutic Drug Monitoring ELISA for estimation of the drug or Immunogenecity ELISA for quantification of anti-drug-antibodies are well validated and sensitive as the critical source reagents – the coating and detection antibodies used for a majority of […]
Types of ELISA: Sandwich, Competitive, Direct and Indirect There are many ELISA tests for particular molecules that use the matching antibodies. ELISA tests are broken into several types of tests based on how the analytes and antibodies are bonded and used. The major types are described here. Direct ELISA The steps of direct ELISA follows […]
Technical Note: Free, Bound, and Total Drug Estimation of monoclonal antibody drugs Understanding the pharmacokinetic and pharmacodynamic behavior of a given therapeutic drug is an essential element of understanding its effectiveness and safety, as well as identifying the proper dosage, combinations and distribution. All pre-clinical and clinical studies include the measurement of serum drug concentration […]
Product Highlight: Ligand Binding Assays for GLP-1 analogs (Peptide Drugs) in line with FDA Considerations Background: Glucagon-like peptide-1 (GLP-1) analogs also known as incretin mimetics are class of drugs indicated for type-2 diabetes mellitus to lower the blood sugar level. The mechanism of the GLP-1 analogs is to prevent the breakdown of GLP-1. This enhances […]
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