FastNuclease Endonuclease Serratia Marcescens

Krishgen s FastNuclease Enzyme (also referred to as Nuclease, Serratia marcescens` extracellular endonuclease with CAS No.: 9025-65-4) is a recombined Serratia marcescens extracellular endonuclease expressed in Escherichia coli (E. coli). It has a purity of > 95%, as determined by SDS-PAGE and endotoxin levels < 0.22 EU/1000 units as determined by the LAL method. FastNuclease has a predicted N terminal of three isoforms with different N terminal from the compound Sm1 (22D-266N), Sm2 (23T-266N) and Sm3 (25E-266N), the activity analysis shows that they were functionally equivalent. FastNuclease is lyophilized from sterile 50 mM Tris-HCl pH 8.0, 20 mM NaCl, 2 mM MgCl2, 5 % trehalose, 5 % mannitol, and 0.01 % Triton (Triton is a trademark of Union Carbide, USA). Follow the instructions on the vial. Centrifuge the vial at 4 before opening to recover the entire contents. Please contact us for any concerns or special requirements. Major Applications of FastNuclease 1. Cell Lysis and Lysate Clarification- Removes nucleic acids from lysates to reduce viscosity and facilitate protein extraction 2. Protein Purification – Eliminates DNA/RNA contamination from protein prep workflows (e.g., chromatography) 3. Biopharmaceutical Production – Used in recombinant protein, viral vector, and monoclonal antibody manufacturing to reduce host cell DNA to acceptable regulatory limits (e.g., 10 ng/dose per WHO/FDA). The residual FastNuclease can be estimated using our Krishgen FastNuclease Endonuclease Serratia Marcescens ELISA (SKU: KBBA36) 4. Downstream Purification of Vaccines and Gene Therapies – Clears nucleic acid contaminants from AAV, lentiviral, and adenoviral vector preps 5. Enzyme or Antibody Prep for ELISA / Western Blot – Reduces background by eliminating nucleic acid-bound proteins 6. Sample Prep for Electrophoresis / Chromatography – Prevents smearing and viscosity during SDS-PAGE, SEC, or HPLC 7. Flow Cytometry Sample Prep – Helps reduce clumping of nuclei-rich samples for better cell suspension 8. Cell-Free Expression Systems – Used to clear residual template DNA/RNA from transcription-translation reactions