Introduction:
The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be
serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA
technique which leads to a higher specificity and increased sensitivity compared to conventional competitive
ELISA kits which employ only one antibody. Double antigens are used in this kit.
Intended Use:
The GENLISA Anti-Hamster Reovirus 3 IgG, REO ELISA is used as an analytical tool for qualitative
determination of Anti-Hamster Reovirus 3 IgG, REO in serum, plasma and other biological samples.
Principle:
The method employs sandwich ELISA technique. Hamster Reovirus 3 antigen is pre-coated onto microwells.
Samples and controls are pipetted into microwells and Anti-Hamster Reovirus 3 IgG, REO present in the sample
are bound by the antigens. Then a Horseradish Peroxidase (HRP)-antigen conjugated is pipetted and incubated
to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution
(TMB) is added to microwells and color develops proportionally to the amount of Anti-Hamster Reovirus 3 IgG,
REO ELISA in the sample. Color development is then stopped by addition of stop solution. Absorbance is
measured at 450 nm.
If you have published a paper by using any of our ELISA since 01/01/2023, kindly fill out the “Krishgen Publication Reward Application Form” with complete information and send it by at email: info@krishgen.com, with the subject “Krishgen Publication Reward”. We will get back to you with the Amazon / Krishgen Credit Reward after we confirm it ASAP!
