Introduction:
The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be
serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique
which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits
which employ only one antibody. Double antigens are used in this kit.
Intended Use:
The GENLISA Hamster Pneumonia Virus, PVM Antibody IgG ELISA is used as an analytical tool for qualitative
determination of Hamster Pneumonia Virus PVM Antibody IgG in serum, plasma and other biological samples.
Principle:
The method employs sandwich ELISA technique. Hamster Pneumonia Virus PVM antigen is pre-coated onto
microwells. Samples and controls are pipetted into microwells and Hamster Pneumonia Virus PVM Antibody IgG
present in the sample are bound by the antigens. Then a Horseradish Peroxidase (HRP)-antigen conjugated is
pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding,
the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Hamster
Pneumonia Virus PVM Antibody IgG ELISA in the sample. Color development is then stopped by addition of stop
solution. Absorbance is measured at 450 nm.
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