Introduction:
The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.
Intended Use:
Mouse TNF- ELISA is specifically designed for the accurate quantitation of Mouse TNF- from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
Principle:
The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and Standards are pipetted into microwells and Mouse TNF- present in the sample is bound by the antibodies. After washing Biotin labeled antibody is added and incubated. After washing Streptavidin-HRP is pipetted and incubated. Washing removes any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Mouse TNF- present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm
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