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₹10,000.00 ₹28,000.00
GST Applicable
Enzyme Immunoassay for the estimation of Interleukin 1 Beta (IL-1B / IL1B) in serum, plasma, cell culture supernatant and other biological samples.
Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells, break-apart wells, break-apart wells
ELISA Features include:
– Ready to use protocol with breakapart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision
Validated against seven points for a”GOLD RING”Standard Quality ELISA – the benchmark sign for Krishgen quality.
The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma, biological fluids and cell culture supernatant.
| Pack Type | 1 x 96 wells, break-apart wells |
|---|---|
| Species Reactivity | Human |
| Sample Type | Serum, Plasma, Cell Culture Supernatant and other biological samples. Validated for citrated/EDTA Plasma only. |
| Assay Range | 15.6 – 1, 000 pg/ml |
| Sensitivity | 5.7 pg/ml |
| Interference | Preparations of the known factors were assayed for interference. No significant interference was observed. |
| Specificity | This assay recognises both the recombinant and natural protein for – IL1-B; IL1-Beta; IL1F2; IL-1Beta; Interleukin-1 Family Member 2; Catabolin |
| Principle Of Assay | This ELISA is a sandwich immunoassay . Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples. |
| Detection Method | Colorimetric, Absorbance measured at 450nm |
| Conjugate-Enzyme Reaction | Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development. |
| Quality Certification | Validated as per KRISHGEN's Quality Program |
| KRISHGEN Mandate | High Performance Assays at Affordable Prices |
| Regulatory Status | Research Use Only. |
| Shipping Temperature | Room Temperature / 2-8 degrees Celsius |
| Storage Temperature | The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity. |
| ELISA Type | Sandwich ELISA, Double Antibody |
| ELISA Interpretation | Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results. |
| Shelf Life Available | 8 -10 months at time of shipping |
| Alternate Names / Synonyms | IL1-B; IL1-Beta; IL1F2; IL-1Beta; Interleukin-1 Family Member 2; Catabolin |
| Research Area | Immune molecule |
| Disclaimer | The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit. |
