PrecisionBind Human Interleukin 1 Beta (IL-1B / IL1b) ELISA (NIBSC/WHO calibrated)

Name: PrecisionBind Human Interleukin 1 Beta (IL-1B / IL1b) ELISA (NIBSC/WHO calibrated)
SKU: kb1063
Availability: InStock

SKU: kb1063

Rs 38,000.00

Enzyme Immunoassay for the estimation of Interleukin 1 Beta (IL-1B / IL1b) in human serum, plasma, cerebrospinal fluid and other biological samples.

PrecisionBind ELISA are matrix-evaluated cytokine immunoassays designed for immune monitoring and translational research, with reference-standard calibration (NIBSC/WHO where available) and performance documentation aligned to applied bioanalysis (ICH M12 for linearity, recovery, precision, and matrix effects). RUO only.

Sample matrices Validated: Serum, Plasma, Cerebrospinal Fluid, Cell Culture Supernatant and Cell / Tissue Lysates

Assay Range: 3.9 – 250 pg/ml | Sensitivity: 0.1 pg/ml

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells, break-apart

9 in stock

The precisionbind Human Interleukin 1 Beta (IL-1B / IL1b) ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Principle Of Assay: This ELISA is a sandwich immunoassay. Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.

Disclaimer: The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit.

Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. The The precisionbind ELISA kits are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of Interleukin 1 Beta (IL-1B / IL1b);

About PrecisionBind:
PrecisionBind is Krishgen`s cytokine ELISA platform developed for bioanalytical robustness in translational research, including immuno-oncology and cell & gene therapy programs. Where applicable, kit standards are calibrated against NIBSC/WHO international standards to support unit traceability and inter-study comparability.

Assays are characterized following ligand-binding assay performance expectations, including:

Defined assay range and sensitivity (LOD/LOQ where reported)

Intra- and inter-assay precision

Cross-matrix performance characterization (serum, plasma, CSF where applicable), including parallelism and recovery assessment, to support quantitative comparability across biological matrices.

All kits are Research Use Only (RUO) and not intended for diagnostic use.

Citations

Additional information

Pack Type	1 x 96 wells, break-apart
Species Reactivity	Homo Sapiens (Human)
Sample Type	cell lysates, cerebrospional cell culture supernates and other biological fluids. K2EDTA serum and plasma validated only. For tear samples, plasma, please connect with us to discuss further., Serum, tissue homogenates
Short Name	Interleukin 1 Beta (IL-1B / IL1b) ELISA
Assay Range	3.9 – 250 pg/ml
Standards Available	0; 3.906; 7.8125; 15.625; 31.25; 62.5; 125; 250 pg/ml
Analytical Sensitivity: LOD	0.1 pg/ml
Functional Sensitivity: LOQ	0.3 pg/ml
IC50%	~266 pg/ml
Calibration Curve	8 point; standards available
Interference	Preparations of the known factors were assayed for interference. No significant interference was observed.
Specificity	The assay uses monoclonal antibody against the analyte and is highly specific as a capture protein.
Principle Of Assay	Double Antibody, Sandwich ELISA
Detection Method	Absorbance measured at 450nm, Colorimetric
Conjugate-Enzyme Reaction	Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.
Regulatory Status	Research Use Only.
Shipping Temperature	Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius
Storage Temperature	if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results
ELISA Interpretation	4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, Quantitative Results Intepretation, to support interpretation of results.
Shelf Life Available	8 -10 months at time of shipping
Alternate Names / Synonyms	Interleukin 1 Beta; IL-1B; IL1b; IL-1 Protein; IL-1B Protein; IL1-BETA Protein; IL1F2
Research Area	Cell Biology; Immunology; Gene Therapy, Cell Therapy
Disclaimer	The data indicated herein with specifications are changed from time to time at time of production. Please refer the most current IFU (instructions for use) accompanying the kit.