Introduction: BNP32 (Nesiritide/Natrecor) is an endogenous peptide secreted from cardiac ventricles in response to volume increase and pressure overload that acts as an agonist at atrial natriuretic peptide (ANP) receptor A (NRP1). In vitro: BNP 1-32 activats cGMP. BNP significantly inhibited de novo collagen synthesis as assessed by [3H]proline incorporation, whereas zymographic MMP-2 (gelatinase) abundance was significantly stimulated by BNP between 10(-7) and 10(-6) mol/L. Decreases de novo collagen synthesis and increases MMP gene expression in vitro. In vivo: inhibits the sympathetic and reninangiotensin-aldosterone systems. Intended Use: The BNP32 ELISA is used for estimation of BNP32 in serum, plasma, or tissue extracts in pharmacokinetics, peptide delivery study and other purposes. Principle: The BNP32 ELISA is a sandwich immunoassay for the determination of BNP32. The antibodies coated on a 96-well plate capture the standard or sample. BNP32 antiserum is subsequently bound to this complex. Biotin is then added followed by SA-HRP (streptavidin-conjugated horseradish peroxidase), which produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific BNP32 bound.
KRIBIOLISA BNP 32 (Human) (Natrecor) ELISA
SKU: KBI5002
Rs 76,000.00
Immunoassay for quantitative estimation of BNP32 in human serum, plasma and tissue extracts. About the ELISA: – Uses highly specific monoclonal antibodies with low cross reactivity – Recovery rates are between 85 – 115% – Ready to use with a standard protocol with break-apart pre-coated wells – Validated as per US FDA guidelines for Bioassays and well optimized – Shelf life: 1 year
Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells
Additional information
| Target / Pathway | Therapeutic target / pathway (unspecified) |
|---|---|
| Active Molecule / Modality | Biologic Drug / Protein |
| Pack Type | 1 x 96 wells, break-apart |
| Species Reactivity | Homo Sapiens (Human) |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. For tear samples, please connect with us to discuss further. Note the kit has been validated using human matrices only. For any other animal matrix, pleas connect with us to optimize the kit accordingly. |
| Analyte / Drug | Human |
| Species / Population Reported for Cmax Value | not available |
| Dose & Regimen Reported | not available |
| Cmax (value + units) | Not reported in label/PI |
| Cmax Source (DOI/URL), where available | not available |
| Sample Dilution | Samples are to be diluted 1:1000 with the Sample Diluent provided in the kit. For more specific dilution instructions, please refer to the most updated IFU enclosed with the kit supplied |
| Assay Range Correlated with Cmax Value, where available | 0 – 2000 ng/ml |
| Standards Available | 0; 31.25; 62.5; 125; 250; 500; 1000; 2000 ng/ml |
| Calibration Curve | 6/8 point; standards available |
| Sensitivity | 15 ng/ml |
| Interference | Preparations of the known factors were assayed for interference. No significant interference was observed. |
| Specificity | The assay uses anti idiotypic (humanized) antibody for capture and / or recombinant protein expressed in mamalian cells, The standards used are research grade biosimilars referenced to the innovator product. In specfic cases where vailable the kit has also been validated against the innovator / reference product. Refer our detailed validation guide for the same. |
| Detection Method | Colorimetric, Absorbance measured at 450nm |
| Conjugate-Enzyme Reaction | Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development. |
| Shipping Temperature | Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius |
| Storage Temperature | The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity. |
| ELISA Type | Sandwich ELISA, Double Antibody |
| ELISA Interpretation | Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results. |
| Shelf Life Available | 8 -10 months at time of shipping |
| Alternate Names / Synonyms | BNP 32; Human; Natrecor |
| Research Area | Immune Molecule; Cell Biology; Immunology, Tumor |
