The KRIBIOLISA Bioprocess Assay Kits available enable researchers to estimate and measure the amount of residual analyte left post downstream purification process. These assays can help identify or characterize the residual analyte which is important in early process and final product release.
This Assay kit is designed to facilitate the identification and characterization of bioprocess analytes used in production and purification.
Assay Features include:
– Ready to use protocol with pre-coated breakapart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision
Validated against seven points for a Gold Ring Standard Quality – the benchmark sign for Krishgen quality.
The kits are used for assessing the specific biomarker in samples analyte which may be cell culture supernatant and other biological preparations.
Unlike competitor assays available, we offer pre-coated ready-to-use assays to ensure high level of reproducibility and accuracy and also offer custom assay development which is process specific to comply with FDA needs from our ISO13485 certified factory and labs.
Protocol
Assay Procedure:
Bring all reagents to room temperature prior to use. It is strongly recommended that all standards and samples be run in duplicate or triplicate. A standard curve is required for each assay.
1. Pipette 100 ul of Standards or Samples into the respective wells.
2. Cover the plate and incubate at 37 Degree Celcius for 2 hours 30 minutes.
3. Aspirate and wash plate 4 times with Wash Buffer (1X) and blot residual buffer by firmly tapping plate upside down on absorbent paper. Wipe of any liquid from the bottom outside of the microtiter wells as any residue can interfere in the reading step.
4. Add 100 ul of Anti-P.pastoris:HRP Conjugate into each well.
5. Cover the plate and incubate at 37 Degree Celcius for 1 hour.
6. Aspirate and wash plate 4 times with Wash Buffer (1X) and blot residual buffer by firmly tapping plate upside down on absorbent paper. Wipe of any liquid from the bottom outside of the microtiter wells as any residue can interfere in the reading step.
7. Add 100 ul of TMB Substrate in each well.
8. Incubate the plate at 37 Degree Celcius for 15 minutes in dark. DO NOT SHAKE or else it may result in higher backgrounds and worse precision. Positive wells should turn bluish in color.
9. Pipette out 100 ul of Stop Solution. Wells should turn from blue to yellow in color.
10. Read the absorbance at 450 nm with a microplate reader within 30 minutes.
