mRNA Cap 2 -O-methyltransferase was derived from a recombinant E. coli strain that carries the gene for the vaccinia mRNA Cap 2-O-Methyltransferase. This enzyme adds a methyl group at the 2-O position of the first nucleotide adjacent to the cap structure at the 5 end of the RNA. The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped RNA (cap-0) resulting in a cap-1 structure. The Cap 1 structure can increase the translation efficiency, improving the expression of mRNA in transfection and microinjection experiments. This enzyme specifically requires RNA with an m7GpppN cap as substrate. It cannot utilize RNA with pN, ppN, pppN or GpppN at the 5 end. Capped RNA may be prepared via in vitro transcription using cap analog or through enzymatic capping using the Vaccinia CappingEnzyme
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KRISHZYME™ mRNA Cap2-O-Methyltransferase
SKU: KLPL10701-2-3
mRNA Cap 2 -O-methyltransferase was derived from a recombinant E. coli strain that carries the gene for the vaccinia mRNA Cap 2-O-Methyltransferase. This enzyme adds a methyl group at the 2-O position of the first nucleotide adjacent to the cap structure at the 5 end of the RNA. The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped RNA (cap-0) resulting in a cap-1 structure. The Cap 1 structure can increase the translation efficiency, improving the expression of mRNA in transfection and microinjection experiments. This enzyme specifically requires RNA with an m7GpppN cap as substrate. It cannot utilize RNA with pN, ppN, pppN or GpppN at the 5 end. Capped RNA may be prepared via in vitro transcription using cap analog or through enzymatic capping using the Vaccinia CappingEnzyme.
Availability: 3 – 4 weeks | Pack Size: 1 x 96 wells
Additional information
| Calibration Range | Refer Kitinsert |
|---|---|
| Sensitivity | Refer Kitinsert |
| Regulatory Status | Research Use Only |
| Storage Temperature | Store it under sterile conditions at -20C to -80C |
