Krishzyme Pyrophosphatase Inorganic (yeast) is derived from a recombinant E. coli strain carrying Saccharomyces cerevisiae ppa gene. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate. P207 4 + H20 2HP04 2 Pyrophosphatase Inorganic (yeast) could hydrolyze the inorganic pyrophosphate generated by nucleic acid amplification, in vitro transcription and other reactions, removing the inhibition of the inorganic pyrophosphate generated on the reaction system. The removal of pyrophosphate can shift the reaction equilibrium to the end of product formation, which is beneficial to increase the yield of synthetic product. RNA and DNA synthesis are examples of reactions that can be pulled far in the synthesis direction by the action of inorganic pyrophosphatase.
Unit Definition
One unit is the amount of enzyme that will generate 1 mol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions. Standard reaction conditions: a 10-minute reaction at 25 C in 100 mM Tris-HCl, [pH 7.2], 2 mM MgCl2 and 2 mM PPi in a reaction volume of 0.5 ml.
Molecular Weight
The molecular weight of Pyrophosphatase, Inorganic (yeast) is 71 kDa.
Application
Krishzyme Pyrophosphatase Inorganic (yeast) enhances yields of RNA in transcription reactions