Murine RNase inhibitor is a recombinant murine RNase inhibitor expressed and purified from E.coli. It binds to RNase A, B or C in a 1:1 ratio through non-covalent bonding, thereby inhibiting the activity of the three enzymes and protecting RNA from degradation. However, It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. Murine RNase inhibitor was tested by RT-PCR, RT-qPCR and IVT mRNA, and was compatible with various commercial Reverse transcriptases, DNA polymerases and RNA polymerases. Compared to human RNase inhibitors, murine RNase inhibitor does not contain two cysteines that are highly sensitive to oxidation which causes inactivation of the inhibitor. That makes it stable at low concentrations of DTT (less than 1 mM). This feature makes it suitable for use in reactions where high concentrations of DTT is adverse to the reaction (eg., Real-time RT-PCR).
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KRISHZYME™ RNase Inhibitor, murine
SKU: KLPL10401-2-3
Murine RNase inhibitor is a recombinant murine RNase inhibitor expressed and purified from E.coli. It binds to RNase A, B or C in a 1:1 ratio through non-covalent bonding, thereby inhibiting the activity of the three enzymes and protecting RNA from degradation. However, It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. Murine RNase inhibitor was tested by RT-PCR, RT-qPCR and IVT mRNA, and was compatible with various commercial Reverse transcriptases, DNA polymerases and RNA polymerases. Compared to human RNase inhibitors, murine RNase inhibitor does not contain two cysteines that are highly sensitive to oxidation which causes inactivation of the inhibitor. That makes it stable at low concentrations of DTT (less than 1 mM). This feature makes it suitable for use in reactions where high concentrations of DTT is adverse to the reaction (eg., Real-time RT-PCR)
Availability: 3 – 4 weeks | Pack Size: 1 x 96 wells
Additional information
| Calibration Range | Refer Kitinsert |
|---|---|
| Sensitivity | Refer Kitinsert |
| Regulatory Status | Research Use Only |
| Storage Temperature | Store it under sterile conditions at -20C to -80C |
