Introduction: Tetanus is a medical condition characterized by a prolonged contraction of skeletal muscle fibers. The primary symptoms are caused by tetanospasmin, a neurotoxin produced by the Gram-positive, rod-shaped, obligate anaerobic bacterium Clostridium tetani. Intended Use: This kit is used to assay the Tetanus Antibody (Tetanus Ab) in the sample of mouse serum and blood plasma. Principle: In Mouse Anti-Tetanus Toxoid ELISA, Tetanus Toxoid (TT) is pre-coated in microwell. Addition of Standard or Samples containing Mouse Tetanus Antibody (Anti-TT Ab) reacts with pre-coated Tetanus Toxoid and form a Immune complex in microwell. Followed by addition of HRP Conjugate and incubation, (TT)- (Anti-TT Ab)-( HRP Conjugate) immune complex will be formed, which is directly proportional to the concentration of (Anti-TT Ab) .Upon washing, unbound HRP Conjugate will be removed. TMB substrate is added to the wells. The amount of hydrolyzed substrate is read on a microplate reader and it is directly proportional to the concentration of Tetanus Antibody (Anti-TT Ab) present in standard or sample.
Additional information
| Sample Type | Mouse Serum, plasma |
|---|---|
| Calibration Range | 3.75 – 120 ng/ml |
| Storage Temperature | Store all main kit components at 2-8 Degree Celcius |
| Species | Mouse |
| Regulatory Status | Research Use Only available |
| Detection Method | Colorimetric |
