Introduction:
In lactating cattle, the Milk Ring Test can be used for screening herds for brucellosis. The test is performedon bulk tank milk samples. If necessary, samples could be pre-treated with preservative. A strongly positive reaction is indicated by formation of a dark blue ring above a white milk column. Any blue layer at the interface of milk and cream should be considered to be positive as it might be significant, especially in large herds. The test is considered to be negative if the color of the underlying milk exceeds that of the cream layer..
Intended Use:
The QUALICHEK Brucella Agglutination Milk Ring Test Kit can be used for screening herds to detect brucellosis in lactating cows.
Principle:
S-brucella antigen is agglutinated by homologous antibodies in milk of cows sick with brucellosis or vaccinated with agglutinogenic brucellosis vaccines. The antigen-antibody complex is absorbed by milk fat globules and forms a colored cream layer at the top. Brucella serum contains specific agglutinins. It is used as positive control in Brucella milk ring test.
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