• Mycoplasma Testing

Mycoplasma Testing

Mycoplasma contamination is a significant problem to the culture of cells / cell lines used for research. Mycoplasma can cause devastating effects on eukaryotic cells as they can modify the cellular parameter leading to variable experimental results and potentially unsafe biological products. Mycoplasmas cannot be detected by visual inspection using a normal light microscope and thus, can remain unnoticed in the cell cultures for long periods. This makes the testing for Mycoplasma essential to ensure reliable research, reproducibility of data, and to meet regulatory requirements.

The test for mycoplasma should be performed at least once per individual sample matrix and must be repeated whenever there is a change in the composition that may affect the detection of mycoplasmas.

Krishgen offers mycoplasma detection services in cell culture supernatant samples. Kindly note, this testing service is only available for our customers in India. It is not available for customers outside the country.

 

SPECIFICITY: The primer used in this assay amplifies the highly conserved 16S rRNA region of the Mycoplasma genome. The detection spectrum includes:

  • M. orale,
  • M. fermentans,
  • M. hyorhinis,
  • M. argininii,
  • M. salivarium,
  • M. hominis,
  • Acholeplasma laidlawii,

and many other species. However, this assay does not detect the clinically relevant species M.pneumoniae and Ureaplasma urealyticum, which are not known as cell culture contaminants. Eukaryotic DNA is not amplified.

HAVE SAMPLES READY? Results in 3 – 5 days. Get a quote.

 

Key Features:

  • Superior technical expertise.
  • Sensitive test
  • Reliable and cost effective testing.
  • Quick turn around time.
  • Tests carried out as per specific guidelines.
  • Advanced data analysis software, data storage and analysis.
  • Well-equipped PCR laboratory with fully automated sample testing systems.

 

RECOMMENDED SAMPLE MATERIAL: Culture suspension or adherent cells, primary cells, virus stocks and other biological can be tested. Culture supernatants are the preferred sample material. Polymerase Chain Reaction is the most commonly used technique used for detecting Mycoplasma. The Mycoplasma DNA is isolated and amplified in vitro by DNA polymerase using specific primers to generate adequate amount of target, which is visualized on the agarose gel for detection. A successful PCR reaction is indicated by a distinct 479 bp band derived from the internal control DNA on the agarose gel.

Extra charges are applicable if the samples need additional pre-treatment.

DETECTION LIMIT: The detection limit is approx. 10-15 fg of Mycoplasma DNA.

REPORTING FORMAT: The test is qualitative, and the results are reported as Detected or Not Detected. A PDF will be emailed upon completion, and a hard copy is available on request.

TURN AROUND TIME: The results are reported 5-7 days from receipt of the samples.

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