Peptide Drug Antibody / Peptibody ELISA
Peptide drugs have emerged as a promising class of therapeutics, offering unique advantages in precision medicine. Peptides have key attributes making them ideal drug candidates including high selectivity and potency – allowing them to interact with specific cellular targets with remarkable accuracy. Along with predictable metabolism, high tolerability, and standard manufacturing processes, they quicky became popular therapeutic targets. By modulating key biological processes, peptide drugs have demonstrated significant potential in treating a diverse range of diseases, including cancer, metabolic disorders, cardiovascular conditions, and autoimmune disorders.
Peptide therapeutics are typically associated with lower production complexity compared with protein-based biopharmaceuticals and the production costs are lower. Approximately 140 peptide therapeutics are currently being evaluated in clinical trials. KRISHGEN today offers the largest range of assays to measure these peptide drugs for potency and PK studies.
- validated as per EMA/FDA guidelines in line with ICH Code for Harmonization of Biological Assays.
- Produced in an ISO 13485 certified facility.
- Validated for human serum and plasma samples.
- Optimised to minimise lot-to-lot variation
- Developed using highly specific antibodies with many assays having unique competitive inhibition assays with proprietary stabilizers and blockers for optimal noise:signal ratio.
- Recovery: 85 – 120%
- Lyophilized standards allow for greater stability.
- One of the only commercially available assays in the world for these peptide targets.
- Many assays use unique pathway linked inhibition assays in competitive format.
- Qualified assays in sandwich formats to offer higher specificity and sensitivity.
- Can be customised for animal serum or plasma to suit your sample type.
Sensitivity: The limit of detection is defined as the lowest detectable concentration corresponding to a signal of Mean of ‘0’ standard plus 2* SD. Each kit mentions sensitivity in the datasheet.
Calibration: The Standards provided in the kit are calibrated against commercially sourced innovator peptide drugs. Where available NIBSC validated standards have been used for calibration of the standards.
Recovery and Matrix Effect: The kits have been optimized at specific dilution to ensure optimal noise:signal ratios. Please do not change the dilution of samples recommended in the IFUs. For any queries, please connect with us directly.
Precision: Precision is defined as the percent coefficient of variation (%CV) i.e. standard deviation divided by the mean and multiplied by 100. Assay precision was determined by both intra and inter assay reproducibility on two pools with low, medium, and high concentrations. While actual precision may vary from laboratory to laboratory and technician to technician, it is recommended that all operators achieve precision below these design goals before reporting results.
World’s Largest Range of Peptide Drug ELISA
Krishgen’s range of Peptide Drug ELISA for quantitative estimation of peptide drugs in samples are the first such assays in the world, and for many assays, we are the only company manufacturing them worldwide. Krishgen Peptide Drug ELISA are sensitive, specific and well validated. They can also be customized upon request.
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Data Reduction: We typically use linear graphs and 4PL (2nd order) as the data reduction method. Cubic spline is also recommended in our IFUs. Our typical graph shows the graph in 4PL and also indicates the R2 value with the trend line shown.
Peptide Drug Antibody / Peptibody ELISA
There are many different prospects for the use of peptide therapeutics. The simplest is peptide replacement. Some diseases are due to the body producing a defunct peptide or not producing it at all, for instance, insulin production in diabetes, therefore simply providing the correct functioning peptide is sufficient for the treatment. Some types of peptide drug functions include:
- Receptor Modulation: Peptide drugs can selectively bind to specific receptors on cell surfaces, initiating a cascade of signalling events. By targeting receptors involved in disease processes, such as hormone receptors or growth factor receptors, peptide drugs can either activate or inhibit these pathways, leading to therapeutic outcomes. This receptor-mediated approach allows for precise targeting and modulation of specific cellular functions.
- Enzyme Inhibition: Peptide drugs can act as enzyme inhibitors by binding to and blocking the activity of specific enzymes involved in disease processes. For example, protease inhibitors, such as HIV protease inhibitors, interfere with viral replication by inhibiting the viral protease enzyme essential for the maturation of new viral particles.
- Protein-Protein Interactions: Peptide drugs can disrupt or modulate protein-protein interactions critical for disease progression. By interfering with specific protein interactions, peptide drugs can prevent the formation of harmful complexes or disrupt aberrant signalling pathways. This approach holds promise for conditions such as cancer, where targeting protein-protein interactions can inhibit tumor growth and metastasis.
- Immuno-modulation: Peptide drugs can influence immune responses and regulate immune system function. They can act as immuno-modulators by stimulating or suppressing specific immune cells, modulating cytokine production, or influencing immune cell trafficking. Peptide-based immunotherapies, such as immune checkpoint inhibitors, have revolutionized cancer treatment by enhancing the body’s immune response against tumor cells.
- Cell Penetration and Intracellular Delivery: Certain peptide drugs possess cell-penetrating properties, allowing them to cross cell membranes and reach intracellular targets. These peptides can facilitate the delivery of therapeutic cargo, such as small molecules or nucleic acids, into cells, enabling targeted and efficient drug delivery to specific cellular compartments.
- Hormonal Regulation: Peptide hormones, such as insulin and glucagon, play essential roles in regulating various physiological processes. Peptide hormone-based therapies can restore hormonal balance or compensate for hormone deficiencies. By mimicking or modulating the activity of endogenous hormones, peptide drugs can effectively regulate metabolic, endocrine, and reproductive functions.
- Tissue Targeting: Peptide drugs can be designed to exhibit tissue-specific targeting, allowing them to selectively accumulate in diseased tissues or target specific cells. This targeted approach minimizes off-target effects and enhances the therapeutic efficacy of the drug.
If you are unable to locate your desired ELISA kit on our website, or have a technical query, please drop us a message and our team will come back to you with availability. If we do not have it available, we will suggest a feasible custom development option.
Require a Custom Assay?
Krishgen can develop and manufacture to your specific requirements.
Whether you require a change in the assay range or sensitivity, want an assay validated for a specific matrix or even want an novel immunoassay developed, the Krishgen team can support you. Get a free technical consultation to learn about the feasibility and timeline of your new assay.