Description
Background:
Abelacimab is a monoclonal antibody that binds to the catalytic domain of Factor XI with high affinity and locks it in a zymogen conformation, thereby preventing its activation by Factor XIIa or thrombin. Abelacimab was designed to prevent the activation of circulating (zymogen) FXI, thereby preventing this coagulation factor from entering the thrombogenic process, as well as inhibit any FXIa that has already been formed.
Intended Use:
For Estimation of Abelacimab in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Abelacimab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Abelacimab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Abelacimab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Abelacimab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
If you have published a paper by using any of our ELISA since 01/01/2023, kindly fill out the “Krishgen Publication Reward Application Form” with complete information and send it by at email: info@krishgen.com, with the subject “Krishgen Publication Reward”. We will get back to you with the Amazon / Krishgen Credit Reward after we confirm it ASAP!