KRIBIOLISA™ Dasatinib (SPRYCEL) ELISA

SKU: KOD1016 Category:

67,500.00

Enzyme Immunoassay for the quantitative determination of Dasatinib in human serum and plasma.

About the ELISA:
– Uses highly specific monoclonal antibodies with low cross reactivity
– Recovery rates are between 85 – 115%
– Ready to use with a standard protocol with break-apart pre-coated wells
– Validated as per US FDA guidelines for Bioassays and well optimized
– Shelf life: 1 year


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells



Description

Introduction:
Dasatinib, sold under the brand name Sprycel, is a targeted therapy medication used to treat certain cases of chronic myelogenous leukemia (CML) and acute lymphoblastic leukemia (ALL). Specifically it is used to treat cases that are Philadelphia chromosome-positive (Ph+). It is a tyrosine-kinase inhibitor and works by blocking a number of tyrosine kinases such as Bcr-Abl and the Src kinase family.

Intended Use:
The KRIBIOLISA Dasatinib ELISA kit is used for quantitative estimation of Dasatinib in human serum and plasma. The kit is for research use only and not for diagnostic use.

Principle:
The Dasatinib ELISA is a competitive inhibition immunoassay for the determination of Dasatinib. A varying concentration of unlabeled Dasatinib Standard or diluted Sample, constant concentration of ABL1 protein and biotin conjugated Anti-ABL1 are added and incubated to the microtitre plate coated with Anti-ABL1. Dasatinib inhibits the binding of Anti-ABL1 to ABL1 protein. Streptavidin:HRP conjugate is added and incubated. Upon washing, unbound Strep:HRP Conjugate will be removed. Bound Streptavidin:HRP conjugate complex will produce a soluble blue colored product after the addition of TMB Substrate. The enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of bound Dasatinib present in the standards or samples.










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Additional information

Species

Human

Sample Type

Human Serum and plasma

Calibration Range

0 pg/ml – 12, 000 pg/ml

Sensitivity

1 nM

Detection Method

Colorimetric

ELISA Type

Direct Sandwich Assay

Regulatory Status

For Research Use Only

Research Area

Cancer

Storage Temperature

Store the unopened product at 2-8 Deg C. Do not use past expiration date.

Validation

The kit uses a biosimilar research grade standard / calibrator. Please refer the IFU for more details.

Disclaimer

Trade name indicated is for reference purposes only. It does not reflect any licences or patent usage. The tradename is the registered trademark of the respective owners only.