KRIBIOLISA™ Degarelix (Firmagon) ELISA

SKU: KBI5008 Category:

47,000.00

Immunoassay for quantitative estimation of Degarelix in human serum, plasma and cell culture supernatant.

About the ELISA:
– Uses highly specific monoclonal antibodies with low cross reactivity
– Recovery rates are between 85 – 115%
– Ready to use with a standard protocol with break-apart pre-coated wells
– Validated as per US FDA guidelines for Bioassays and well optimized
– Shelf life: 1 year


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells



Description

Introduction:
Degarelix is used for the treatment of advanced prostate cancer. Degarelix is a synthetic peptide derivative drug which binds to gonadotropin-releasing hormone (GnRH) receptors in the pituitary gland and blocks interaction with GnRH. This antagonism reduces luteinizing hormone (LH) and follicle-stimulating hormone (FSH) which ultimately causes testosterone suppression. Reduction in testosterone is important in treating men with advanced prostate cancer. FDA approved the same as & ldquo;Firmagon& rdquo; in December 2008.

Intended Use:
The KRIBIOLISA Degarelix (Firmagon) ELISA is used to estimate the level of Degarelix in human serum and plasma. The kit is intended for research use only.

Principle:
The ELISA is a competitive immunoassay for the determination of Degarelix. A constant concentration of Degarelix coated on the microplate and varying concentration of standard or sample will compete for binding to gonadotropin-releasing hormone (GnRH) receptor. Detection antibody against GnRH conjugated to HRP is added to form a complex. The complex will produce a soluble colored product on substrate addition. The enzyme reaction is stopped by dispensing of Stop Solution into the wells. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of bound Degarelix present in standards or samples.










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Additional information

Species

Human

Sample Type

Precoated plates using monoclonal antibodies; Sample type -Serum, cell cuture supernatant and plasma

Calibration Range

0- 2000 ng/ml

Sensitivity

40ng/ml

Detection Method

Colorimetric

ELISA Type

Direct Sandwich Assay

Regulatory Status

For Research Use Only

Research Area

Cancer

Storage Temperature

Store the unopened product at 2-8 Deg C. Do not use past expiration date.

Validation

The kit uses a biosimilar research grade standard / calibrator. Please refer the IFU for more details.

Disclaimer

Trade name indicated is for reference purposes only. It does not reflect any licences or patent usage. The tradename is the registered trademark of the respective owners only.