Description
Introduction:
Desmopressin (Minirin, Nocdurna, DDAVP) is a medication used to treat diabetes insipidus, bedwetting, hemophilia A, von Willebrand disease, and high blood urea levels. Desmopressin (dDAVP), a synthetic analogue of 8-arginine vasopressin (ADH), is an antidiuretic peptide drug modified by deamination of 1- cysteine and substitution of 8-L-arginine by 8-D-arginine. ADH is an endogenous pituitary hormone that has a crucial role in the control of the water content in the body. Upon release from the stimulation of increased plasma osmolarity or decreased circulating blood volume, ADH mainly acts on the cells of the distal part of the nephron and the collecting tubules in the kidney. The hormone interacts with V1, V2 or V3 receptors with differing signal cascade systems.
Intended Use:
The KRIBIOLISA Desmopressin (Minirin, Nocdurna, DDAVP) ELISA is used as an analytical tool for the quantitative determination of desmopressin in human serum and plasma.
Principle:
The KRIBIOLISA Desmopressin (Minirin, Nocdurna, DDAVP) is a competitive inhibition immunoassay for the determination of Desmopressin. Anti-DDAVP monoclonal antibody are pre-coated onto the microwells. A constant concentration of Biotin Concentrate and varying concentrations of Standard or Sample compete for binding / inhibiting specifically to the coated antibodies. Then, Streptavidin:HRP conjugate is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops in inverse proportion to the amount of Desmopressin in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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