KRIBIOLISA™ Desmopressin (Minirin) ELISA

SKU: KBI5010 Category:

47,000.00

Enzyme Immunoassay for the quantitative determination of Desmopressin in human serum and plasma.

About the ELISA:
– Uses highly specific monoclonal antibodies with low cross reactivity
– Recovery rates are between 85 – 115%
– Ready to use with a standard protocol with break-apart pre-coated wells
– Validated as per US FDA guidelines for Bioassays and well optimized
– Shelf life: 1 year

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells



Description

Introduction:
Desmopressin (Minirin, Nocdurna, DDAVP) is a medication used to treat diabetes insipidus, bedwetting, hemophilia A, von Willebrand disease, and high blood urea levels. Desmopressin (dDAVP), a synthetic analogue of 8-arginine vasopressin (ADH), is an antidiuretic peptide drug modified by deamination of 1- cysteine and substitution of 8-L-arginine by 8-D-arginine. ADH is an endogenous pituitary hormone that has a crucial role in the control of the water content in the body. Upon release from the stimulation of increased plasma osmolarity or decreased circulating blood volume, ADH mainly acts on the cells of the distal part of the nephron and the collecting tubules in the kidney. The hormone interacts with V1, V2 or V3 receptors with differing signal cascade systems.

Intended Use:
The KRIBIOLISA Desmopressin (Minirin, Nocdurna, DDAVP) ELISA is used as an analytical tool for the quantitative determination of desmopressin in human serum and plasma.

Principle:
The KRIBIOLISA Desmopressin (Minirin, Nocdurna, DDAVP) is a competitive inhibition immunoassay for the determination of Desmopressin. Anti-DDAVP monoclonal antibody are pre-coated onto the microwells. A constant concentration of Biotin Concentrate and varying concentrations of Standard or Sample compete for binding / inhibiting specifically to the coated antibodies. Then, Streptavidin:HRP conjugate is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops in inverse proportion to the amount of Desmopressin in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

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Additional information

Species

Human

Sample Type

Precoated plates using monoclonal antibodies; Sample type – Serum, Plasma, or Tissue extracts

Calibration Range

0.1-100 ng/ml

Detection Method

Colorimetric

ELISA Type

Direct Sandwich Assay

Regulatory Status

For Research Use Only

Research Area

Diabetes / Metabolism

Storage Temperature

Store the unopened product at 2-8 Deg C. Do not use past expiration date.

Validation

The kit uses a biosimilar research grade standard / calibrator. Please refer the IFU for more details.

Disclaimer

Trade name indicated is for reference purposes only. It does not reflect any licences or patent usage. The tradename is the registered trademark of the respective owners only.