Description
Background:
Drozitumab is a human monoclonal antibody in development for the treatment of cancers. It targets Tumour Necrosis Factor Related Apoptosis-inducing Ligand (TRAIL), whose receptors are found on the surface of many types of malignant cells. Although drozitumab was studied in phase II trials for treating chondrosarcoma, colorectal cancer, non-Hodgkin lymphoma, and non-small cell lung cancer, development has been halted due to lack of clinical response.
Intended Use:
For Estimation of Drozitumab in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Drozitumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Drozitumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Drozitumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Drozitumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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