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Introduction:
Erythropoietin (EPO) is a heavily glycosylated protein with a molecular weight of about 30,000 – 34,000 Daltons. EPO is a polypeptide consisting of 166 amino acids, containing one O-linked and three N-linked carbohydrate chains. The recombinant EPO is a good substitute for the native protein for use in an immunoassay.
Intended Use:
This kit is used for the quantitative determination of EPO (Erythropoietin) in serum, plasma and cell culture supernatant
Principle:
The method employs sandwich ELISA technique. Streptavidin is pre-coated onto microwells. Samples and standards are pipetted into microwells and EPO present in the sample are bound by the biotin labeled antibodies to the microwell. Anti-EPO-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of EPO in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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