Description
Background:
Elgemtumab, also known as LJM716, is a high- affinity IgG1 type monoclonal antibodies selected from a Human Combinatorial Antibody Library (HuCAL). It can specifically bind to ERBB3 to neutralize multiple modes of ERBB3 activation. ERBB3 is the abbreviation of receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3). It is a member of the ERBB family of receptor tyrosine kinases (RTK). ERBB3 mediated activation of PI3K signaling when inappropriate ERBB2/ERBB3 dimerization, which usually happens in HER2 or neuregulin (NRG) over-expression cancer cells. Thus, ERBB3 is consideration as a mediator of oncogenic transformation and a therapeutic target of cancer. Elgemtumab can potently inhibit the phosphorylation of ERBB3/ AKT and the proliferation of tumor cells over-expressing HER2 and NRG.
Intended Use:
For Estimation of Elgemtumab in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Elgemtumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Elgemtumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Elgemtumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Elgemtumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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