Description
Background:
Garetosmab is a human IgG4 anti-Activin A antibody under investigation for the management of fibrodysplasia ossificans progressiva. Fibrodysplasia ossificans progressiva (FOP) is a rare autosomal dominant disorder of episodic, progressive heterotropic ossification. FOP is characterized by episodic inflammatory episodes, which may be precipitated by trauma, including immunizations and minor tissue damage, which usually result in ossification of the lesion.Patients experience abnormal cartilage formation, growth plate dysplasia, and joint issues, resulting in progressive immobility and associated comorbidities.The discovery of an activating mutation in the ACVR1 receptor that renders it responsive to the (normally antagonistic) Activin A led to an interest in Activin A as a therapeutic target
Intended Use:
For Estimation of Garetosmab in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Garetosmab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Garetosmab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Garetosmab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Garetosmab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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