Description
Introduction:
The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. There is no standard reference SARS-CoV-2 antigen material available; accordingly, absolute analytical sensitivity cannot be calculated. The kit uses a polyclonal antibody as Positive Control. The assay allows the qualitative determination of samples.
Intended Use:
The GENLISA Human Anti-SARS-CoV-2 (Covid-19) IgG ELISA kit is used as an analytical tool for qualitative estimation of IgG antibodies to Human SARS-CoV-2 (Covid-19) in human serum and plasma, respiratory specimens and cell culture supernatant.
Principle:
The method employs sandwich ELISA technique. Human SARS-CoV-2 protein is pre-coated onto microwells. Samples and controls are pipetted into microwells and IgG Antibodies to human SARS-CoV-2 (Covid-19) present in the sample are bound by the protein antigen. After incubation the wells are washed and followed by HRP-conjugated Detection IgG Antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to the microwells. Color develops proportionally to the amount of IgG Anti-Human SARS-CoV-2 (Covid-19) in the samples. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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