GENLISA™ Human SARS-CoV-2 (Covid-19) Spike RBD Omicron Variant (B.1.1.529) Antigen Quantitative TITRATION ELISA

SKU: KBVH015-48 Categories: ,

48,000.00

ELISA for the quantitative detection of SARS-CoV-2 spike protein RBD of the Omicron Variant (B.1.1.529) in human serum and plasma samples. Lead time is 2 – 3 weeks. IFU / Datasheet coming soon.


Availability: 3 – 4 weeks | Pack Size: 1 x 96 wells



Description

Introduction:
The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.

Intended Use:
The GENLISA Human SARS-CoV-2 (Covid-19) Spike RBD Omicron Variant (B.1.1.529) Antigen Quantitative TITRATION ELISA kit is used as an analytical tool for quantitative antigen determination of Human SARS-CoV-2 (Covid-19) Spike RBD Omicron Variant (B.1.1.529) in human serum and plasma and cell culture supernatant.

Principle:
The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells.Samples and standards are pipetted into microwells and Human SARS-CoV-2 (Covid-19) Spike RBD Omicron Variant (B.1.1.529) present in the sample are bound by the capture antibodies. After incubation the wells are washed and followed by HRP- conjugated Detection Antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human SARS-CoV-2 (Covid-19) Spike RBD Omicron Variant (B.1.1.529) in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.









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Additional information

Species

Human

Sample Type

Serum and Plasma

Calibration Range

62.5 – 4000 ng/ml

Detection Method

Colorimetric, 450nm

Regulatory Status

For Research Use Only

Research Area

COVID-19