KRIBIOLISA™ Ipilimumab (YERVOY) ELISA

SKU: KBI1025 Category:

66,000.00

Enzyme Immunoassay for the estimation of Ipilimumab (YERVOY) in serum and plasma.

Key features:
1. Developed using highly specific monoclonal anti-idiotypic antibodies in a sandwich assay format
2. Validated as per international bioassay validation guidelines
3. Recovery: 80-120%
4. Precision CV <10%

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells



Description

Background:
Ipilimumab is a first-in-class T-cell potentiator that works by blocking CTLA-4, a critical negative regulator of the anti-tumor T-cell response. In response to full T-cell activation, CTLA-4 is upregulated and competes with CD28 for CD80 and CD86 binding on antigen presenting cells but with significantly higher affinity, therefore downregulatingor deactivatingthe T cells. CTLA-4, therefore, downregulates T-cell responses and antigen presenting cell function, resulting in a decreased immune response to tumor associated antigens and immune tolerance. A fully humanized IgG1 monoclonal antibody, by blocking CTLA-4, Ipilimumab removes an inhibitory signal from reducing the activity of T lymphocytes.

Intended Use:
For Estimation of Ipilimumab (YERVOY) in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.

Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Ipilimumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Ipilimumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Human IgG antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Ipilimumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

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Additional information

Sample Type

Serum (100 ul), EDTA Plasma (100 ul), Heparin Plasma (100 ul), Citrate Plasma (100 ul)

Calibration Range

20 ng/ml – 640 ng/ml

Detection Method

Colorimetric

Specificity

80% – 120% to Ipilimumab (YERVOY)

Cross Reactivity

< 0.5% cross-reactivity observed with related biomarker.

Interference

No significant interference observed with available related molecules.

Regulatory Status

Research Use Only

Research Area

Immune Checkpoints

Entez Gene ID

BMS-734016 Human

ELISA Type

Direct Sandwich Assay

Storage Temperature

Store the unopened product at 2-8 Deg C. Opened products to be stored as per temperatures indicated on the datasheet.

Validation

The kit uses a biosimilar research grade standard / calibrator. The standard / calibrator has been validated against WHO/NIBSC standard and/or against commercially sourced innovator drug where available. Please refer to the IFU for more details.

Disclaimer

Trade name indicated is for reference purposes only. It does not reflect any licences or patent usage. The tradename is the registered trademark of the respective owners only.

Long Name

Protein Based Therapies – Monoclonal Antibody