KRISHZYME™ Hyaluronidase Enzymatic Assay Kit

SKU: KBBA05 Category:

52,000.00

Enzymatic Assay for Quantification of Hyaluronidase activity in cell culture supernatant and pharmaceutical preparations.

About the kit:
? Uses high quality reagents from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
? Recovery rates are between 85 ? 115%
? Ready to use with a standard protocol with break-apart pre-coated wells
? Validated as per US FDA guidelines for Bioassays
? Optimized for matrix effects to ensure higher sensitivity.
? Shelf life: 1 year


Availability: 3 – 4 Weeks | Pack Size: 2 x 96 wells



Description

The KRISHZYME Hyaluronidase Assay kit uses a two-step turbidimetric reaction to measure hyaluronidase activity by the amount of hyaluronic acid that is hydrolyzed. A stop reagent halts the enzymatic reaction and forms turbidity with any residual hyaluronic acid in the well. The decrease in turbidity at 600 nm is directly proportional to hyaluronidase activity in the sample.

Hyaluronidase is a family of enzymes that degrade hyaluronic acid by catalyzing the hydrolysis of hyaluronan, a constituent of the interstitial barrier. Hyaluronidase lowers the viscosity of hyaluronan, thereby increasing tissue permeability. It is, therefore, used in medicine in conjunction with other drugs to speed their dispersion and delivery.










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Additional information

Sample Type

Cell Culture Supernatant and Pharmaceutical Preparations

Detection Method

Colorimetric

Regulatory Status

For Research Use Only

Calibration Range

28 U/ml – 896 U/ml

Storage Temperature

Store the unopened product at 2-8 Deg C. Do not use past expiration date.