KRIBIOLISA™ Lixisenatide ELISA

SKU: KBI5021 Category:

47,000.00

Immunoassay for quantitative estimation of Lixisenatide in human serum, plasma and tissue extracts.

About the ELISA:
– Uses highly specific monoclonal antibodies with low cross reactivity
– Recovery rates are between 85 – 115%
– Ready to use with a standard protocol with break-apart pre-coated wells
– Validated as per US FDA guidelines for Bioassays and well optimized
– Shelf life: 1 year


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells



Description

Introduction:
Lixisenatide (Lyxumia) is a recombinant DNA produced polypeptide analogue of human glucagon-like peptide1 (GLP-1) which is used in combination with diet and exercise in the therapy of type 2 diabetes, either alone or in combination with other antidiabetic agents.

Intended Use:
The Lixisenatide ELISA is used for estimation of Lixisenatide in serum, plasma, or tissue extracts in pharmacokinetics, peptide delivery study and other purposes.

Principle:
The Lixisenatide ELISA is a competitive immunoassay for the determination of Lixisenatide. The antiserum is captured by antibodies coated on a 96-well plate. A constant concentration of Biotin Concentrate and varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum. Captured Bt-tracer is subsequently bound by SA-HRP (streptavidin-conjugated horseradish peroxidase), which produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of specific Lixisenatide bound.










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Additional information

Species

Human

Sample Type

Serum, Plasma, or Tissue extracts

Calibration Range

0 – 12000 ng/ml

Sensitivity

~180 ng/ml

Detection Method

Colorimetric

ELISA Type

Direct Sandwich Assay

Regulatory Status

For Research Use Only

Research Area

Diabetes / Metabolism

Storage Temperature

Store the unopened product at 2-8 Deg C. Do not use past expiration date.

Validation

The kit uses a biosimilar research grade standard / calibrator. Please refer the IFU for more details.

Disclaimer

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