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Introduction:
The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody.
Intended Use:
The GENLISA SARS-CoV-2 (2019-nCoV) Spike S1 Antibody Quantitative ELISA (Mouse) kit is used as an analytical tool for quantitative estimation of Anti-SARS-CoV-2 (2019-nCoV) Spike S1 antibodies in mouse serum.
Principle:
The method employs indirect sandwich ELISA technique. SARS-CoV-2 Spike S1 recombinant protein is precoated onto microwells. Samples and standards are pipetted into microwells and Antibodies to Mouse AntiSARS-CoV-2 (2019-nCoV) present in the sample are bound by the protein antigen. After incubation the wells are washed and followed by addition of HRP-conjugated Detection IgG Antibody into each well and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of AntiMouse Anti-SARS-CoV-2 S1 (2019-nCoV) in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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