Description
Introduction:
A variety of antibodies are used as therapeutics agent as well as in pharmaceuticals. These antibodies are generally purified through protein A column chromatography. This method of purification has the potential with contamination of protein A, which might get leached out of the column and co-eluted with purified antibody. This may result in adverse toxic or immunological reactions that ultimately affect the efficacy of the product antibody. The highly sensitive Protein A ELISA is a quantitative, simple and will aid in monitoring the purification process as well as screening product prior to its use.
Intended Use:
This kit is used for determination of natural and recombinant constructs of Protein A ligand contamination, in various antibody products that are purified through Protein A chromatographic techniques.
Principle:
This assay is based on the principle of Sandwich ELISA. Samples containing Protein A are reacted with antiProtein A antibody coated on the microtitre plates. The wells are washed to remove any unbound reactants as per the wash procedure. Anti-Protein A HRP antibody is added to the wells and incubated. . The wells are washed to remove any unbound reactants as per the wash procedure. simultaneously in the microtiter wells already coated with affinity purified capture anti-Protein A antibody. The substrate Tetramethyl Benzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the concentration of Protein A present.
If you have published a paper by using any of our ELISA since 01/01/2023, kindly fill out the “Krishgen Publication Reward Application Form” with complete information and send it by at email: info@krishgen.com, with the subject “Krishgen Publication Reward”. We will get back to you with the Amazon / Krishgen Credit Reward after we confirm it ASAP!