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Introduction:
The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody.
Intended Use:
The GENLISA Rat Anti-SARS-CoV-2 (Covid-19) IgG Antibody to spike RBD protein Quantitative TITRATION ELISA kit is used as an analytical tool for quantitative estimation of Anti-SARS-CoV-2 (2019-nCoV) spike RBD antibodies in rat serum.
Principle:
The method employs indirect sandwich ELISA technique. SARS-CoV-2 Spike RBD protein is pre-coated onto microwells. Samples and standards are pipetted into microwells and Antibodies to Rat Anti-SARS-CoV-2 (2019-nCoV) present in the sample are bound by the protein antigen. After incubation the wells are washedand followed by addition of HRP-conjugated Detection IgG Antibody into each well and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Anti-Rat Anti-SARS-CoV-2 (2019-nCoV) in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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