KRIBIOLISA™ Teriparatide (PTH -34) ELISA

SKU: KBI5032 Category:

47,000.00

Immunoassay for quantitative estimation of Teriparatide in human serum, plasma and tissue extracts.

About the ELISA:
– Uses highly specific monoclonal antibodies with low cross reactivity
– Recovery rates are between 85 – 115%
– Ready to use with a standard protocol with break-apart pre-coated wells
– Validated as per US FDA guidelines for Bioassays and well optimized
– Shelf life: 1 year

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells



Description

Introduction:
Teriparatide is a recombinant protein form of parathyroid hormone consisting of the first (N-terminus) 34 amino acids, which is the bioactive portion of the hormone. It is an effective anabolic (i.e., bone growing) agent used in the treatment of some forms of osteoporosis. The Teriparatide ELISA kit is designed to measure teriparatide with high specificity while minimizing crossreactivity to human PTH (1-84) and other PTHrPs.

Intended Use:
The Teriparatide ELISA is used for estimation of Teriparatide in serum, plasma, or tissue extracts in pharmacokinetics, peptide delivery study and other purposes.

Principle:
The Teriparatide ELISA is a competitive immunoassay for the determination of Teriparatide. The antiserum is captured by antibodies coated on a 96-well plate. A constant concentration of Biotin Concentrate and varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum. Captured Bt-tracer is subsequently bound by SA-HRP (streptavidin-conjugated horseradish peroxidase), which produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of specific Teriparatide bound.

If you have published a paper by using any of our ELISA since 01/01/2023, kindly fill out the “Krishgen Publication Reward Application Form” with complete information and send it by at email: info@krishgen.com, with the subject “Krishgen Publication Reward”. We will get back to you with the Amazon / Krishgen Credit Reward after we confirm it ASAP!

View more details about our Publication Reward

Additional information

Species

Human

Sample Type

Precoated plates using monoclonal antibodies; Sample type – Serum, Plasma, or Tissue extracts

Calibration Range

0 – 640 ng/ml

Sensitivity

~9.5 ng/ml

Detection Method

Colorimetric

ELISA Type

Direct Sandwich Assay

Regulatory Status

For Research Use Only

Storage Temperature

Store the unopened product at 2-8 Deg C. Do not use past expiration date.

Validation

The kit uses a biosimilar research grade standard / calibrator. Please refer the IFU for more details.

Disclaimer

Trade name indicated is for reference purposes only. It does not reflect any licences or patent usage. The tradename is the registered trademark of the respective owners only.