Description
Background:
Abrezekimab (UCB4144) is an anti-IL-13 agent that is in development as an anti-asthmatic agent. IL23 immune signal, preventing intestinal inflammation.
Intended Use:
For Estimation of Abrezekimab in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Abrezekimab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Abrezekimab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Abrezekimab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Abrezekimab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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