KRIBIOLISA™ Ampicillin ELISA

SKU: KRA1002 Category:

67,000.00

Enzyme Immunoassay for quantitative estimation of Ampicillin in cell culture supernatant and other purified systems including vaccines.

Other features include:
– Standardization and Reproducibility
– Lot to Lot consistency
– Accuracy and Precision to add confidence to your results
– Validated against seven points for a gold ring standard quality ELISA designed as per the US FDA guidelines for bioassays, a marker for Krishgen quality.
– Long shelf life and break-apart wells


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells



Description

Background: Ampicillin belongs to the beta-lactams family, which once played very important role in preventing and curing animal diseases. For it causes anaphylactic reaction and resistance, in EU, US and China, it is being restricted. The common instrumental analysis of this drug is limited because of the complicated operation and high expense, while this kit is a new product based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 1.5h in single detection, so it can considerably minimize operation error and work intensity.This ELISA can be used in the production of therapeutic drugs and vaccines, to accurately quantify drug residues and ensure quality and purity of the final product. Current method of drug residue testing such as HPLC or GC-MS are with sample prep not only complex and expensive, but also difficult and time consuming. ELISAs speed up and simplify the analysis without losing accuracy.

Principle: KRIBIOLISA Ampicillin ELISA kit is based on indirect-competitive ELISA technology. The microtiter wells are coated with Ampicillin antigen. Ampicillin residue in the sample competes with the antigen coated on the microtiter plate for the antibody. After the addition of enzyme labeled anti-antibody, TMB substrate is used to show the color. Absorbance of the sample is negatively related to the ampicillin reside in it, after comparing with the Standard Curve, multiplied by the dilution factor, ampicillin residue quantity in the sample can be calculated.









If you have published a paper by using any of our ELISA since 01/01/2023, kindly fill out the “Krishgen Publication Reward Application Form” with complete information and send it by at email: info@krishgen.com, with the subject “Krishgen Publication Reward”. We will get back to you with the Amazon / Krishgen Credit Reward after we confirm it ASAP!

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Additional information

Sample Type

Cell Culture Supernatant and Other Biological Preparations

Calibration Range

0.2 ng/ml – 16.2 ng/ml

Sensitivity

0.2 ng/ml

Detection Method

Colorimetric

Cross Reactivity

Ampicillin – 100%, Penicillin – 52%, Penicillin V – < 20

ELISA Type

Indirect Competitive

Regulatory Status

For Research Use Only

Storage Temperature

Store the unopened product at 2-8 Deg C. Do not use past expiration date.