Description
Background:
Aprutumab is a fully human FGFR2 monoclonal antibody, which binds to the FGFR2 isoforms FGFR2-IIIb and FGFR2-IIIc. Aprutumab has the potential for solid tumors research as antibody drug conjugate Aprutumab Ixadotin.
Intended Use:
For Estimation of Aprutumab in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Aprutumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Aprutumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Aprutumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Aprutumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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