Description
Background:
Aprutumab Ixadotin is an antibody-drug conjugate (ADC) directed against the fibroblast growth factor receptor type 2 (FGFR2) and conjugated to an as of yet unidentified toxin, with potential antineoplastic activity. Upon intravenous administration, aprutumab ixadotin binds to FGFR2. Upon binding, the toxin selectively induces cell death, through an as of yet undisclosed mechanism of action, in FGFR2-expressing tumor cells. FGFR2, a receptor tyrosine kinase upregulated in many tumor cell types, plays an essential role in tumor cell proliferation, differentiation and survival.
Intended Use:
For Estimation of Aprutumab Ixadotin in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Aprutumab Ixadotin are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Aprutumab Ixadotin present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Aprutumab Ixadotin antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Aprutumab Ixadotin in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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