KRIBIOLISA™ Atezolizumab (TECENTRIQ) ELISA
₹66,000.00
Enzyme Immunoassay for the estimation of Atezolizumab (TECENTRIQ) in serum and plasma.
Key features:
1. Developed using highly specific monoclonal anti-idiotypic antibodies in a sandwich assay format
2. Validated as per international bioassay validation guidelines
3. Recovery: 80-120%
4. Precision CV <10%
Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells
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Description
Background:
Atezolizumab (trade name Tecentriq) is a fully humanized, engineered monoclonal antibody of IgG1 isotype against the protein programmed cell death-ligand 1 (PD-L1). In 2015, it was in clinical trials as an immunotherapy for several types of solid tumors. It was under investigation by Genentech/Roche. In April 2016, Roche announced that atezolizumab had been granted fast track status for lung cancer by the FDA. In May 2018, Tecentriq was in combination with Avastin and standard chemotherapy for some patients with lung cancer was granted priority review.
Intended Use:
For Estimation of Atezolizumab (TECENTRIQ) in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Atezolizumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Atezolizumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Atezolizumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Atezolizumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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Additional information
| Calibration Range | 20 ng/ml ? 640 ng/ml |
|---|---|
| Sensitivity | 80 ng/ml |
| Detection Method | Colorimetric |
| Specificity | 80% – 120% to Atezolizumab (TECENTRIQ) |
| Cross Reactivity | < 0.5% cross-reactivity observed with related biomarker. |
| Interference | No significant interference observed with available related molecules. |
| Storage Temprature | Store the unopened product at 2-8 Degree Celcius. Concentrated detection conjugate should be stored at -20 Degree Celcius. Do not use past expiration date. |
| Shipping Temperature | 2-8 Degree Celcius. Concentrated detection conjugate ship at -20 Degree Celcius in a second box. Freight applicable for both. |
| Regulatory Status | Research Use Only |
| Research Area | Immune Checkpoints |
| Entez Gene ID | MPDL 3280A Human |
| Alternate Names / Synonyms | Atezolizumab |
| Alternate Drug Brand Names | Tecentriq |
| Drug Bank Accession Number | DB11595 |
| ELISA Type | Direct Sandwich Assay |