Description
Background:
Brodalumab has been used in trials studying the treatment of Asthma, Psoriasis, Crohn’s Disease, Psoriatic Arthritis, and Rheumatoid Arthritis. Brodalumab was FDA approved in February, 2017 as Siliq for the treatment of moderate-to-severe plaque psoriasis.
Intended Use:
For Estimation of Brodalumab (SILIQ) in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Brodalumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Brodalumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Brodalumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Brodalumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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