Description
Background:
Camidanlumab (HuMax-TAC) is a CD25 monoclonal antibody. Camidanlumab targets the cell-surface antigen CD25, which is over-expressed on a variety of hematological tumors and shows limited expression on normal tissues. Camidanlumab can be used for synthesis of antibody agent conjugates (ADCs) to research several diseases including lymphoma and leukemia. It is designed as an antibody drug conjugate Camidanlumab Tesirine.
Intended Use:
For Estimation of Camidanlumab Tesirine in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Camidanlumab Tesirine are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Camidanlumab Tesirine present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Camidanlumab Tesirine antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Camidanlumab Tesirine in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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